Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis

Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis

Xylose reductase (XR) is the first enzyme in D: -xylose metabolism, catalyzing the reduction of D: -xylose to xylitol. Formation of XR in the yeast Candida tropicalis is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose. This is one reason why glucose is not a suitable co-substrate for cell growth in i...

Production of xylitol from D-xylose by a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis.

Xylitol dehydrogenase (XDH) is one of the key enzymes in d-xylose metabolism, catalyzing the oxidation of xylitol to d-xylulose. Two copies of the XYL2 gene encoding XDH in the diploid yeast Candida tropicalis were sequentially disrupted using the Ura-blasting method. The XYL2-disrupted mutant, BSXDH-3, did not grow on a minimal medium containing d-xylose as a sole carbon source. An enzyme assa...

Optimized Production of Xylitol from Xylose Using a Hyper-Acidophilic Candida tropicalis

The yeast Candida tropicalis DSM 7524 produces xylitol, a natural, low-calorie sweetener, by fermentation of xylose. In order to increase xylitol production rate during the submerged fermentation process, some parameters-substrate (xylose) concentration, pH, aeration rate, temperature and fermentation strategy-have been optimized. The maximum xylitol yield reached at 60-80 g/L initial xylose co...

Enhanced Xylitol Production from Statistically Optimized Fermentation of Cotton Stalk Hydrolysate by Immobilized Candida tropicalis

Cotton (Gossypium hirsutum), which is one of the most abundant crops in the world, is cultivated widely in China, the United States, and Central Asia. The cotton stalk generated with cotton cultivation is an important source of lignocellulosic biomass. In recent years, cotton stalk has received increasing attention from researchers engaged in bioconversion areas, and some high-value products, s...

Constitutive Expression of an Endogenous Xylose Reductase Gene in Candida tropicalis by Using a Promoter of Glyceraldehyde-3-Phosphate Dehydrogenase